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- Preparation of Stock Solution of Kanamycin (50 mg ml)
Follow the table to prepare a kanamycin solution of different concentrations and volumes
- Kanamycin Stock Solution - GoldBio
Prewet a 0 22 μm syringe filter by drawing through 5-10 ml of sterile H2O and discard water Sterilize Kanamycin Stock through the prepared 0 22 μm syringe filter Stock may be kept at -20°C for 1 year
- How to make a 50 mg ml Kanamycin Stock Solution - protocols. io
Add 10 ml of sterile H2O Disolve completely Prewet a 0 2 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water Sterilize Kanamycin Stock through the prepared 0 2 µm syringe filter Stock may be kept at -20°C for 1 year
- Kanamycin Stock Solution Preparation and Recipe
Prepare 0 8 L of distilled water in a suitable container Add 50 g of Kanamycin sulfate to the solution Filter sterilize the solution into a falcon tube using a 20ml syringe and a 0 22 μm filter Aliquot into 1 5ml Eppendorf tubes Keep stock at -20°C Working Conc = 50 µg mL
- Kanamycin Sulfate - Inhibitors - Products - UBPBio
Kanamycin sulfate is an aminoglycosidic antibiotic that inhibits the growth of Gram-positive and Gram-negative bacteria and mycoplasmas in cell culture Preparation of 1000X stock at 50 mg ml: 1) Dissolve 5 grams of Kanamycin sulfate in 100 ml distilled water
- K0879pis. doc - MilliporeSigma
Prepare stock solutions directly in the vial with sterile water (10 mg ml) Kanamycin sulfate is soluble in water (50 mg ml), yielding a clear solution Kanamycin sulfate is practically insoluble in alcohol, acetone, chloroform, ether, and ethyl acetate A 1% solution in water has a pH of 6 5 to 8 5
- General Zamanian Lab Recipes - Zamanian Lab Docs
Aliquot 1 mL each into pre-labeled 1 5 mL tubes and store at room temperature in labeled box Additional tubes can be stored at -20°C Store at room temperature or at +4°C
- Kanamycin
The ampicillin is prepared at 50 mg mL The protocol to prepare it is: 1 Dissolve 0 5g of kanamycin sulfate (CAS 25389-94-0) in water Add water up 10 mL 2 Prewash a 0 22 – 0 45 μm filter with 20 mL of water 3 Put the washed filter over a new sterile tube Then, filter Kanamycin Stock Solution 4 Aliquot in 1,5 mL tubes and store at -20oC
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