- Restriction enzymes DNA ligase (article) | Khan Academy
For this reason, enzymes that leave single-stranded overhangs are said to produce sticky ends Sticky ends are helpful in cloning because they hold two pieces of DNA together so they can be linked by DNA ligase
- Overview: DNA cloning (article) | Khan Academy
A restriction enzyme is a DNA-cutting enzyme that recognizes a specific target sequence and cuts DNA into two pieces at or near that site Many restriction enzymes produce cut ends with short, single-stranded overhangs
- DNA cloning and recombinant DNA (video) | Khan Academy
DNA cloning involves creating identical copies of a specific gene using restriction enzymes to cut the desired gene, pasting it into a plasmid, and inserting it into bacteria like E coli The bacteria replicate the gene and can produce proteins, such as insulin
- Molecular mechanism of DNA replication - Khan Academy
The RNA primers are removed and replaced by DNA through the activity of DNA polymerase I, the other polymerase involved in replication The nicks that remain after the primers are replaced get sealed by the enzyme DNA ligase
- Restriction enzymes (video) | DNA technology | Khan Academy
So normally if this gets cut, then you'll have two sticky ends, they'll float around and then they'll reanneal And we can actually take advantage of the fact that we can have the strands reanneal, and we'll talk about different ways that we can actually use that for medicinal purposes
- Retroviruses (video) | Translation | Khan Academy
And by clipping off those three prime sections, they form these sticky ends because unpaired DNA wants to be paired And integrase has suddenly removed that part
- Nucleic acids (article) | Khan Academy
At the 5’ end, or beginning, of the chain, the 5’ phosphate group of the first nucleotide in the chain sticks out At the other end, called the 3’ end, the 3’ hydroxyl of the last nucleotide added to the chain is exposed
- DNA structure (article) | Khan Academy
DNA is made up of four types of nucleotides: adenine (A), cytosine (C), thymine (T), and guanine (G) Each nucleotide consists of a phosphate group (P), a deoxyribose sugar, and a nitrogenous base
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