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- Metabolic Flux Analysis with 13C-Labeling Experiments
Generating and improving insights into metabolic pathway activity by comparing flux phenotypes under different environmental conditions and physiological states as well as for a variety of carbon sources
- Frontiers | 13C metabolic flux analysis: Classification and . . .
Recently, it has evolved into a method family with great diversity in experiments, analytics, and mathematics In this review, we classify and characterize the various branch of 13 C-MFA from a unified perspective of mathematical modeling
- 13C Metabolic Flux Analysis | SpringerLink
13 C MFA is an experimental approach to compute intracellular flux pattern It involves tracer studies where substrates are labeled with stable isotopes, such as 13 C, and the labeling pattern of metabolites is subsequently measured
- 13C Metabolic Flux Analysis – Institute of Molecular Systems Biology . . .
Our current efforts aim at developing new approaches and implement the necessary software to measure fluxes in complex situations (ie in higehr cells) and measure fluxes at high-throughputs Early methods of flux analysis aimed at quantifying possibly many fluxes in central carbon metabolism
- p13CMFA: Parsimonious 13C metabolic flux analysis - PLOS
As proof of concept, we demonstrate how p 13 CMFA can be used to estimate intracellular flux distributions from 13 C measurements and transcriptomics data We have implemented p 13 CMFA in Iso2Flux, our in-house developed isotopic steady-state 13 C MFA software
- High-resolution 13C metabolic flux analysis - Nature
The presented 13C-MFA implementation is based on a number of inherent assumptions that collectively form the basis for the isotope labeling balances that are solved when calculating metabolic
- A Guide to 13C Metabolic Flux Analysis for the Cancer Biologist
Here Maciek Antoniewicz reviews 13C metabolic flux analysis (MFA) for quantifying intracellular fluxes in cancer cell metabolism Detailed are the fundamentals for experimental design, analytical measurement, and statistical analysis along with the merits and limitations of various tracer approaches
- Overview of 13c Metabolic Flux Analysis - Creative Proteomics
In this paper, the basic principles and experimental design methods of 13C-MFA will be systematically discussed, and the application of bacterial MFA will be described as an example At the same time, the latest research progress in mammalian cell culture will be discussed in depth
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