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- Studying lipolysis in adipocytes by combining siRNA knockdown and . . .
A centrifugation-assisted infection with recombinant adenovirus, on the other hand, enables robust overexpression of ectopic proteins Most importantly, by combining siRNA electroporation with adenovirus infection, simultaneous manipulation of levels of two different proteins can be achieved in differentiated adipocytes
- An siRNA-based method for efficient silencing of gene expression in . . .
In summary, we report an easy and versatile reverse siRNA transfection protocol to achieve specific silencing of gene expression in various models of mature brown and browning-competent white adipocytes, including primary cells
- Studying lipolysis in adipocytes by combining siRNA knockdown and . . .
In this chapter, we provide a review of two methods established for manipulation of protein expression in differentiated mature adipocytes The use of electroporation allows a high-efficiency delivery of siRNA oligos and subsequent knockdown of specific gene expression
- Methods in Cell Biology | Volume 116: Lipid Droplets - ScienceDirect
Chapter 6 - Studying Lipolysis in Adipocytes by Combining siRNA Knockdown and Adenovirus-Mediated Overexpression Approaches Xiaodong Zhang, Bradlee L Heckmann, Jun Liu
- Lipolysis regulates major transcriptional programs in brown adipocytes
Here, the authors provide unbiased insight into the transcriptional network controlled by lipolysis in brown adipocytes, showing that lipolysis is required for much of the thermogenic gene
- Distinct Mechanisms Regulate ATGL-Mediated Adipocyte Lipolysis by Lipid . . .
In this study, we performed small interfering RNA (siRNA)-mediated knockdown and adenovirus-mediated overexpression experiments in 3T3-L1 adipocytes to assess the roles of perilipin 1 and FSP27 in ATGL-mediated lipolysis
- Studying Lipolysis in Adipocytes by Combining siRNA Knockdown and . . .
In this chapter, we provide a review of two methods established for manipulation of protein expression in differentiated mature adipocytes The use of electroporation allows a high-efficiency
- Research Faculty Directory Profile - USF Health
Zhang X, Heckmann BL, Liu J Studying lipolysis in adipocytes by combining siRNA knockdown and adenovirus-mediated overexpression approaches Methods in cell biology 116: 83-105, 2013
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