- 基因后的Cre+及Cre-分别代表什么含义? - 知乎
Cre是一种酶,它可以识别和切割DNA中特定的序列(称为LoxP位点),从而删除、转座或激活基因。 在你所看到的文献中,“Zbtb16-cre+”和“Zbtb16-cre-”指的是两种小鼠,它们分别含有不同版本的“Zbtb16-cre”基因。
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- 「Cre loxp」技术是什么? - 知乎
Cre-loxP的基本原理 Cre(Cyclization Recombination Enzyme)是一种重组酶,来源于P1噬菌体,其基因编码区序列全长1029bp,为38kDa大小的、由343个氨基酸组成的多肽单体蛋白。
- Cre LoxP重组酶系统的工作原理是什么? - 知乎
Cre LoxP重组酶系统包含两个部分:Cre重组酶及其特异识别的LoxP位点。 Cre重组酶(Cyclization Recombination Enzyme)由大肠杆菌噬菌体P1的Cre基因编码,是由343个氨基酸组成的38kD的蛋白质。它不仅具有催化活性,而且与限制酶相似,能够特异性识别loxP位点。 LoxP(locus of X-overP1)位点长为34bp,包括两个13bp的
- 介绍一下cre-loxp重组酶系统原理,cre-loxp重组酶系统有什么优点? - 知乎
Cre-LoxP系统由Cre重组酶和LoxP位点两个部分组成。 Cre重组酶是整合酶家族的一员,于1981年在大肠杆菌噬菌体P1中发现,是最常用的位点特异性DNA重组酶。
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