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- The intratumoral microbiome: Characterization methods and functional . . .
IHC and FISH staining are generally complemented by correlative light and electron microscopic (CLEM) analysis that confirmed the abundance of microbiome in close proximity to the nuclear membrane of cancer cells [7] However, microscopic detection methods provide no data on the metabolic activity of intratumor bacteria
- Intratumoral microbiome: implications for immune modulation and . . .
Recent advancements have revealed the presence of a microbiome within tumor tissues, underscoring the crucial role of the tumor microbiome in the tumor ecosystem This review delves into the characteristics of the intratumoral microbiome, underscoring its dual role in modulating immune responses and its potential to both suppress and promote tumor growth We examine state-of-the-art techniques
- From Light Microscopy to Analytical Scanning Electron Microscopy (SEM . . .
Comparison of volume electron microscopy techniques, for classical transmission EM (TEM) serial sectioning, consecutive ultrathin sections are collected on grids and imaged separately with TEM (a) For TEM-tomography thin sections (0 3–1 µm) are cut with a diamond knife, collected onto a grid, which is tilted relative to the TEM to 70° After registration and back-projection, a tomogram is
- Computational methods and challenges in analyzing intratumoral . . .
The human microbiome is the collection of all the microorganisms, which are non-negligible components of the human body, residing on or within human tissues and biofluids, such as the skin, oral mucosa, lung, and gastrointestinal tract [1] Human microbes are believed to play a broad role in cancer diagnosis, pathogenesis, and treatment by interacting with the host immune system [2]
- Computational methods and challenges in analyzing intratumoral . . .
The human microbiome is the collection of all the microorganisms, which are non-negligible components of the human body, residing on or within human tissues and biofluids, such as the skin, oral mucosa, lung, and gastrointestinal tract [1] Human microbes are believed to play a broad role in cancer diagnosis, pathogenesis, and treatment by interacting with the host immune system [2] The
- Intratumoral microbiome: implications for immune modulation and . . .
SEM High resolution and magnica-tion, High depth of eld Inability to details and observe internal ne structures of complex samples Observe tumor tissues of in the ESCC [15] CLEM High specicity and resolu - tion, Multicolor uorescence imaging Expensive, Complex operation To conrm that bacteria can enter melanoma cells [17] C-FIB SEM High
- Relevance of harmful intratumoral microbiota in cancer progression and . . .
Correlative light and electron microscopy (CLEM) is an effective research tool that combines optical and electron microscopy advantages to precisely locate cells and molecules at high resolution CLEM is commonly employed for localizing intratumoral microbiota due to its wide field of view, dynamic range, and exceptional resolution capabilities
- FIB-SEM Tomography in Biology - SpringerLink
Focused ion beam (FIB) technology was first described in 1974 by Seliger and Fleming [], who could focus an ion beam to a few micrometers Later they demonstrated a scanning ion system with gallium ions that could be focused to 100 nm (review in ref 2) After these initial approaches, the group of Dr Ishitani at Hitachi [2, 3] refined the instruments and used them for sample preparation for
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