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- biochemistry - How do I interpret the results of this DNA gel . . .
This run was meant to be a sort of mock-forensics experiment There is DNA from the "crime scene", "suspect 1", and "suspect 2" There are 3 samples from each, one is untreated, one is digested with EcoRV, and one is digested with PstI
- Can we run PBEh-3c in Gaussian? - Chemistry Stack Exchange
Can someone help with with setting up a calculation to run the new composite scheme PBEh-3c in Gaussian? The code has been implemented in Turbomole and ORCA and it's pretty easy to run it there
- Why concurrent reading instead of mean in titration? [closed]
First! A buret reading should be to the estimated 0 01 mL, the first Insignificant figure not to the gradations of 0 1 mL One titration is one data point If you want to check your overall precision the procedure is to run complete replicate samples on the one sample or [even on simultaneous replicate samples to check on the sampling technique] Since only 2 or 3 replicates are usually run
- What causes the DNA fragments to stop moving in gel electrophoresis?
Also today it is quite common to have the DNA stain already in the gel while the electrophoresis is running (instead of adding a staining solution at the end of the run) This allows to follow the DNA run in "real time"
- kinetics - Calculation of the specific rate constant (k) - Chemistry . . .
3 Suppose we found that the reaction of Red#3 with bleach is 1st order in hypochlorite The observed rate constant ($k_\mathrm {obs}$) for one of the trials was measured to be $\pu {0 00400 s^ {-1}}$ If the concentration of hypochlorite was fixed at $\pu {0 134 M}$, calculate the specific rate constant ($k$) for that trial
- Convergence issue in Gaussian - Chemistry Stack Exchange
Just few suggestions for finding a stationary point You can add some additional angular flexibility into the basis by changing it to, say, 6-31G(2df,p) or even switch to more modern Ahlrichs' def2 bases (Def2SVP to start from) Besides, you'd better use UltraFine integration grid and tight optimization criteria and do geometry optimization and frequency calculation in a single run: Opt=Tight
- Diffusion Streaky bands on TLC plate - Chemistry Stack Exchange
3 These days I am working on a research project where I am supposed to run TLC for samples which are wax extracts from coconut butten nuts using chloroform as the solvent All the samples have been concentrated to 10% from its original volume
- analytical chemistry - How does the Rf value change when changing the . . .
As, mentioned by Jan Increasing the polarity of solvent will result in increase in Rf value as the compound in even for polar or nonpolar compound, this is the rule of thumb in most cases, may be except do exist i don't know about it But for my experience in the laboratory polarity result increase in Rf value
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