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- Primer designing tool - National Center for Biotechnology Information
Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type Help Entrez query (optional) Help Primer specificity stringency Primer must have at least 1 2 3 4 5 6 total mismatches to unintended targets, including
- Primer-Blast results
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target
- National Center for Biotechnology Information
Try "re-search for specific primers" feature on Primer-BLAST result page When only non-specific primers are found, the result page offers the option to re-search for specific primers
- Primer designing tool - National Center for Biotechnology Information
The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction
- Primer-BLAST Archives - NCBI Insights
To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions It uses these to limit the locations of primers The longest sequence is also used as the representative template sequence in the results
- qPrimerDB 2. 0: an updated comprehensive gene-specific qPCR primer . . .
High-quality primer design is essential for the success of all polymerase chain reaction (PCR)-based experiments We previously developed a thermodynamics-based gene-specific quantitative PCR (qPCR) primer database for 147 organisms, which has been used extensively in gene expression studies
- Primer-Blast results
Primer-BLAST A tool for finding specific primers Making primers specific to your PCR template more Follow NCBI Connect with NLM
- Primer-BLAST: a tool to design target-specific primers for . . . - PubMed
We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers Primer-BLAST offers flexible options to adjust the specificity threshold and other primer properties
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