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'PRIMER_LEFT_EXPLAIN': 'considered 600, too many Ns 148, GC content failed 2, low tm 444, ok 6'} EDIT: I use a while loop to keep designing primers if there are no primers for the target region
- The Definitive C++ Book Guide and List - Stack Overflow
This question attempts to collect the few pearls among the dozens of bad C++ books that are published every year Unlike many other programming languages, which are often picked up on the go from
- C++ Primer 5th Edition Exercise 1. 23 Question about the Solution I . . .
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PRIMER_THERMODYNAMIC_PARAMETERS_PATH (string; default primer3_config) This tag specifies the path to the directory that contains all the parameter files used by the thermodynamic approach In Linux, there are two default locations that are tested if this tag is not defined: primer3_config and opt primer3_config
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Thank you for replying I've added a screenshot of my data I can't provide any code for the first plot as it was created using the PRIMER software, which I no longer have access to But the procedures should be similar, I think? Both did square-root transformation (in R the autotransformation is square-root, I verified it when running mds)
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- The Definitive C Book Guide and List - Stack Overflow
A good general introduction and tutorial C Primer Plus (5th Edition) - Stephen Prata (2004) A Book on C - Al Kelley Ira Pohl (1998) The C Book (Free Online) - Mike Banahan, Declan Brady, and Mark Doran (1991) C: How to Program (8th Edition) - Paul Deitel and Harvey M Deitel (2015) Lots of good tips and best practices for beginners
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