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- Primer designing tool - National Center for Biotechnology Information
Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type Help Entrez query (optional) Help Primer specificity stringency Primer must have at least 1 2 3 4 5 6 total mismatches to unintended targets, including
- PrimedRPA: primer design for recombinase polymerase amplification . . .
By using 105 Zika sequences sourced globally, PrimedRPA identified 140 potential primer-probes sets that would bind to Zika independently of the genetic diversity
- Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing
While it is known that the primer choice has a significant influence on the resulting microbial composition, we show that microbial profiles generated using different primer pairs need independent validation of performance
- Critical Evaluation of Two Primers Commonly Used for Amplification of . . .
We compare our proposed formulation to two common alternatives by using linear amplification—providing an assessment that is independent of a reverse primer—and in combination with the 1492 reverse primer (1492r) under the PCR conditions appropriate for making community rRNA gene clone libraries
- sRNAPrimerDB: comprehensive primer design and search web service for . . .
However, only a few tools can be used to develop the optimal primer or probe design for the expression profile of small ncRNAs Here, we developed sRNAPrimerDB, the first automated primer designing and query web service for small ncRNAs
- Performance of 16s rDNA Primer Pairs in the Study of Rhizosphere and . . .
To evaluate the primer efficiency of the selected primer pairs amplifying pure chloroplast DNA (Populus tremula × P alba), we tested all primer pairs in a qPCR set-up
- Primer-Blast results
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target
- Design PCR primers and check them for specificity
Go to the Primer BLAST submission form Enter the target sequence in FASTA format or an accession number of an NCBI nucleotide sequence in the PCR Template section of the form
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