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- Primer designing tool - National Center for Biotechnology Information
Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type Help Entrez query (optional) Help Primer specificity stringency Primer must have at least 1 2 3 4 5 6 total mismatches to unintended targets, including
- National Center for Biotechnology Information
Try "re-search for specific primers" feature on Primer-BLAST result page When only non-specific primers are found, the result page offers the option to re-search for specific primers
- Primer-Blast results
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target
- Primer-BLAST Archives - NCBI Insights
To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions It uses these to limit the locations of primers The longest sequence is also used as the representative template sequence in the results
- Primer-BLAST: a tool to design target-specific primers for . . . - PubMed
We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers Primer-BLAST offers flexible options to adjust the specificity threshold and other primer properties
- Parameters for Successful PCR Primer Design - PubMed
Despite the publication of numerous guidelines, the actual design of many published assays is often unsound: primers lack the claimed specificity, they may have to compete with secondary structures at their binding sites, primer dimer formation may affect the assay's sensitivity or they may bind only within a narrow temperature range
- Primer designing tool - National Center for Biotechnology Information
The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction
- miR-145-5p inhibits the proliferation and migration of bladder cancer . . .
The miR-145-5p reverse transcription primer is 5′-AGTCCAGTTTTCCCAGGAATCCCT-3′ RT-qPCR was performed through the SYBR Premix Ex Taq (Takara Bio, Inc , Otsu, Japan) on an ABI PRISM 7500 Real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Inc )
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