Bidirectional titration of yeast gene expression using a pooled CRISPR . . . Here, we utilize a library approach that is able to simultaneously modulate gene expression in a metabolism-wide manner This library, when coupled with next-generation sequencing, allows for the identification of gene perturbations that would have been missed by classic approaches
A simplified strategy for titrating gene expression reveals new . . . The approach works by performing CRISPRi with a series of mutated single guide RNAs (sgRNAs) that modulate gene expression To evaluate sgRNA mutation strategies, we constructed a library of 5927 sgRNAs targeting 88 genes in Escherichia coli MG1655 and measured the effects on growth rate