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Using harmony in Seurat - The Comprehensive R Archive Network The stimulated PBMC group was treated with interferon beta ## Generate SeuratObject ```r ## Source required data data("pbmc_stim") pbmc <- CreateSeuratObject(counts = cbind(pbmc stim, pbmc ctrl), project = "PBMC", min cells = 5) ## Separate conditions pbmc@meta data$stim <- c(rep("STIM", ncol(pbmc stim)), rep("CTRL", ncol(pbmc ctrl)))