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Engineering 5-hydroxymethylfurfural (HMF) oxidation in The resulting BOX strains (Boosted OXidation) represent superior hosts for biotechnological synthesis of FDCA from HMF The increased oxidation rates provide greatly elevated HMF tolerance, thus tackling one of the major drawbacks of whole-cell catalysis with this aldehyde
Effective biosynthesis of 2,5-furandicarboxylic acid from 5 . . . As a cost-effective and eco-friendly approach, biocatalysis has great potential for the transformation of 5-hydroxymethylfurfural (HMF) into 2,5-furandicarboxylic acid (FDCA) However, the compatibility of each enzyme in the cascade reaction limits the transformation efficiency of HMF to FDCA
Synthesis of 2,5-furandicarboxylic acid by a TEMPO laccase . . . In this study, we designed a method of coupling reactions to produce FDCA from HMF The key feature of this approach is the oxidation of the hydroxymethyl group via a TEMPO laccase system and the oxidation of the aldehyde moiety by P putida KT2440 cells
Enhanced 2,5-Furandicarboxylic Acid (FDCA) Production in In this study, we report the identification of key genes that are involved in FDCA synthesis and then the engineering of Raoultella ornithinolytica BF60 for biocatalytic oxidation of HMF to FDCA using its resting cells
Gluconobacter oxydans DSM 50049 – an efficient biocatalyst . . . Screening of three different strains showed G oxydans DSM 50049 to possess the highest FFCA oxidation efficiency Optimal reaction conditions for obtaining 100% conversion of 10 g L (71 mM) FFCA to FDCA at 100% reaction yield were at pH 5, 30 °C and using 200 mg wwt mL cells harvested at mild-exponential phase
Efficient Method for Synthesis of 2,5-Furandicarboxylic Acid . . . This catalyst showed excellent catalytic activity toward the synthesis of industrially important 2,5-furandicarboxylic acid (FDCA) from 5-hydroxymethylfurfural (HMF) and fructose The process is simple, efficient, green, and industrially feasible
Enzymatic Cascade for the Synthesis of 2,5‐Furandicarboxylic . . . For the subsequent oxidation of HMF to 2,5-furandicarboxylic acid (FDCA), ‘media-agnostic’ catalysts that can be efficiently used in different conditions, from aqueous to biphasic, and to organic (microaqueous) media, are of interest