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High-Coverage ITS Primers for the DNA-Based Identification of . . . The kingdom Fungi is estimated to include 1 5 million or more species, playing key roles as decomposers, mutualists, and parasites in every biome on the earth To comprehensively understand the diversity and ecology of this huge kingdom, DNA barcoding targeting the internal transcribed spacer (ITS) region of the nuclear ribosomal repeat has been regarded as a prerequisite procedure By