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Cross-linked enzyme aggregates (CLEAs): stable and recyclable . . . In the present review, we describe a novel, versatile and effective methodology for enzyme immobilization as CLEAs (cross-linked enzyme aggregates) The method is exquisitely simple (involving precipitation of the enzyme from aqueous buffer followed by cross-linking of the resulting physical aggregates of enzyme molecules) and amenable to rapid
Cross-linked enzyme aggregate - Wikipedia Cross-Linked Enzyme Aggregates (CLEAs) The use of cross-linked enzyme crystals (CLECs) as industrial biocatalysts was pioneered by Altus Biologics in the 1990s CLECs proved to be significantly more stable to denaturation by heat, organic solvents and proteolysis than the corresponding soluble enzyme or lyophilized (freeze-dried) powder
Characteristic features and biotechnological applications of cross . . . Cross-linked enzyme aggregates (CLEAs) have many economic and environmental benefits in the context of industrial biocatalysis They are easily prepared from crude enzyme extracts, and the costs of (often expensive) carriers are circumvented
CLEAs, Combi-CLEAs and ‘Smart’ Magnetic CLEAs: Biocatalysis in a Bio . . . One method to achieve this is via carrier-free immobilization as cross-linked enzyme aggregates (CLEAs) This methodology proved to be very effective with a broad selection of enzymes, in particular carbohydrate-converting enzymes
An Overview of Crosslinked Enzyme Aggregates: Concept of Development . . . Thus, crosslinked enzyme aggregates (CLEAs) are developed as a better enzyme immobilization tool to extend the enzymes’ operational stability This immobilization method is appealing because it is simpler due to the absence of ballast and permits the collective use of crude enzyme cocktails
Lipase immobilization via cross-linked enzyme . . . - ScienceDirect CLEAs provide lipase stabilization under extreme temperature or pH conditions or in the presence of organic solvents, in addition to preventing enzyme leaching in aqueous medium However, it presents some problems in the preparation and limitations in their use
Cross-linked enzyme aggregates (CLEAs): A novel and versatile method . . . This review describes a novel, versatile and effective methodology for enzyme immobilization, namely, as cross-linked enzyme aggregates (CLEAs) The method is exquisitely simple – involving precipitation of the enzyme from aqueous buffer followed by cross-linking of the resulting physical aggregates of enzyme molecules – and amenable to
Cross-Linked Enzyme Aggregates and Their Application in Enzymatic . . . Carrier-free cross-linked enzyme aggregates (CLEAs) are one of the immobilization methods, which has attracted attention due to ease of preparation and robustness of immobilized enzymes without the need of support (Figure 1)