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Targeted Nanopore Sequencing with Cas9 for studies of . . . Existing capture methods adapted to long read sequencing include CATCH-seq (dual cuts with Cas9 followed by size selection)2, ligation to cuts with a less permissive endonuclease3, cloning the region into an expression plasmid4, region amplification5, and long fragment hybridization capture methods1,6
High throughput targeted metabolomics library generation on a . . . Targeted mass spectrometry (MS)-metabolomics techniques identify and quantify various fecal metabolites, including amino acids, fatty acids, lipids, and bile acids However, traditional MS approaches often face limitations in speed and selectivity, which can restrict the number of metabolites analyzed In response to these challenges, we developed a comprehensive fecal metabolite library using
pH-responsive high stability polymeric nanoparticles for . . . A common strategy for prolonging the half-life of peptides in vivo is to modify them in order to increase their af finity for long-lived serum albumin59,60, the most abundant protein in human
TSD: A computational tool to study the complex structural . . . Targeted sequencing has been widely applied in studies by capturing only the interest region When it comes to long reads, how to use such redundant reads for an accurate SV discovery also challenges the data analysts Here, we present a tool, TSD, for identifying and visualizing the structural variants using PacBio targeted sequencing data
What is targeted proteomics #x0003F; A concise revision of . . . As there is no limitation in which peptides to extract, data-independent acquisition methods at the fragment level can only be considered targeted as long as there is a hypothesis to be tested i e a set of proteins (can be many) are queried to extract their specific quantification information
Targeted long-read sequencing of native DNA for genetic . . . The ability to detect methylation enables simultaneous generation of sequence-level and epigenetic data We present new panel content for the PureTarget assay, which uses the CRISPR Cas9 system to generate targeted sequencing libraries of native DNA, sequenced with long and accurate HiFi sequencing