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Primer designing tool - National Center for Biotechnology Information Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type Help Entrez query (optional) Help Primer specificity stringency Primer must have at least 1 2 3 4 5 6 total mismatches to unintended targets, including
Primer designing tool - National Center for Biotechnology Information Enter the position ranges if you want the primers to be located on the specific sites The positions refer to the base numbers on the plus strand of your template (i e , the "From" position should always be smaller than the "To" position for a given primer) Partial ranges are allowed
Design PCR primers and check them for specificity If the NCBI mRNA reference sequence accession number is used, the tool will automatically design primers that are specific to that splice variant If one or both primer sequences are to be used in the search, enter these in the Primer Parameters section of the form
Primer-Blast results Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target
MPprimer: a program for reliable multiplex PCR primer design The electrophoretic mobility of the amplicons should be considered at the very beginning of primer design In this work, we developed a program named MPprimer to address these issues