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HPLC User Maintenance Troubleshooting - Agilent Pump and Degasser Maintenance Clean the degasser lines by flushing with isopropanol When using buffers, flush with water, then with isopropanol Check for air bubbles in outlet lines Be aware of the possibility of microbial growth in aqueous phases
TROUBLESHOOTING Agilent and Waters HPLC DEGASSER DEGASSING MODULE . . . Connect any unused vacuum channels in-series with one you use, to keep the channels filled at all times (and improve degassing efficiency!) The Waters ALLIANCE degasser components are installed on a removable tray in the pump module You can easily remove this tray as one assembly and send it to us for diagnosis and repair
HPLC Pressure Help : r labrats - Reddit The pressure almost settles out then dips down about once every five minutes I was thinking the degasser may be defective, but I'm not sure as it is affecting all the lines in the system Please let me know if you have any suggestions! Instrument: Agilent 1260 Infinity II w degasser and Binary pump
Waters Acquity UPLC vacuum h w fault degasser error, HPLC Degasser . . . These errors often indicate that your degasser vacuum pump is worn out and or you have vacuum leaks in the tubing or degassing chambers However, before you send in your degasser module to us for diagnostic testing or service, please be sure you review and address the following possible causes of errors
ACQUITY Solvent Manager Error: Degasser vacuum h w failure (1 . . . ACQUITY solvent managers pull down a vacuum for only three minutes upon initialization This amount of time can sometimes be insufficient to effectively purge solvent vapor from the degasser tubing for all mobile phases