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REFERENCE WAVELENGTHS (as used in HPLC UV VIS): REFERENCE WAVELENGTHS (as used in HPLC UHPLC UV VIS): Correct use of the Reference Wavelength in Chromatography (Diode Array Detectors, DAD) is fundamental to obtaining Accurate data
Tips and Tricks for HPLC and UHPLC - Thermo Fisher Scientific Recommended Parameters: Data Acquisition The Program Wizard of Chromeleon has a dedicated step for setting the correct ‘Data Collection Rate’ and ‘Time Constant’ The internal calculation is based on the peak width at half peak height of the slimmest peak in the chromatogram
Optimizing the Bandwidth and Slit Width Settings - Diode Array Detector . . . You can accept the standard bandwidth in Chromeleon, or set the bandwidth to a higher or lower value For a wider or smaller bandwidth, the device can average several single photodiode signals This process is referred to photodiode bunching Note the following:
Wavelength (Reference Information) - J. Everett In Chromeleon configurations without control option, the selected wavelength remains fixed during the analysis In controlled versions, the wavelength can be altered during analysis
Sample and Reference Wavelength and Bandwidth To optimize for lowest possible detectable concentrations of anisic acid, set the sample wavelength to the peak of the absorbance band (that is, 252 nm) and the sample bandwidth to the width of the absorbance band (that is, 30 nm)
Microsoft Word - faq183. doc - Agilent How to select Reference Wavelength If you know little about the analytes in your sample, use both lamps and store all spectra over the full wavelength range The detector measures absorbance simultaneously at wavelengths from 190 to 950 nm Two lamps provide good sensitivity over the whole wavelength range
Reference Wavelength selection - Chromatography Forum I'm trying to assay a drug in plasma extracts by a HPLC system equipped with DAD The analyte has the maximum absorption at 341 nm, and no absorption around 450 nm So I set the DAD condition on 341 nm for detection and 450 nm as the reference wavelenght
HPLC Troubleshooting Guide An App That Will Save Time and Frustration Reference bandwidth serves to average several photodiode signals above and below the reference wavelength A reference bandwidth should be selected as wide as possible, for example 30–100 nm, but narrow enough not to interfere with the absorption spectrum of the analytes
REFERENCE WAVELENGTH, PEAK PURITY, and THRESHOLD In this case, the most popular wavelength is 254 nm, which works great for aromatic compounds The bandwidth is the plus or minus nanometers Instead of just looking at 254 nm, we use 254 ± 8 nm to improve sensitivity and the signal-to-noise ratio Why Do We Use a Reference Wavelength?