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Field Methods — eDNA RESOURCES Peer-reviewed publications usually include summaries of the specific methods used for sample collection, concentration, and preservation, but a handful of published protocols provide more detailed, step-by-step instructions that can be used in many sampling situations
Environmental DNA (eDNA) | U. S. Geological Survey Environmental DNA (eDNA) is organismal DNA that can be found in the environment Environmental DNA originates from cellular material shed by organisms (via skin, excrement, etc ) into aquatic or terrestrial environments that can be sampled and monitored using new molecular methods
Environmental DNA Sampling and Experiment Manual In general, eDNA analysis follows the workflow starting from water sampling through eDNA collection by filtration and eDNA extraction from the filter to the detection of the target species using various molecular techniques
eDNA Sampling Standards and Guidelines Several works have provided detailed eDNA reviews that outline important considerations for specific topics associated with eDNA sampling and analysis, and these can be found in Appendix B (Table B-2)
eDNA Primer - An introductory knowledge base on environmental DNA eDNA allows scientists to examine species quickly and without prior knowledge of which species are present This method can be faster, cheaper, and more effective than other techniques for identifying species in an environment
eDNA sampling for native, threatened and invasive species Where more detailed data is sought about population or individual health (e g , reproductive output, juvenile recruitment, sex, genetic variation, abundance), eDNA sampling can pinpoint sampling locations for more traditional, time-intensive capture methods, thereby reducing survey eforts and costs
Guidance for environmental DNA sampling design and effort vasive species (Lodge et al 2006, Ficetola et al 2008) Initial eDNA efforts attempted to sample in locations with presumably the highest probability of detection if the target species was present, such as downstream from where a species would likely aggregate and where DNA
Environmental DNA Sampling Protocol Filtering Water to Capture DNA from . . . This publication is meant to guide researchers and managers in the collection, concentration, and preservation of eDNA samples from lentic and lotic systems A sampling workflow diagram and three sampling protocols are included as well as a list of suggested supplies