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Western Blot避坑指南：高频问题解析与科学解决方案 - 知乎 Western Blotting (WB)，作为分子生物学实验室的“常青树”技术，是揭示蛋白质表达奥秘的关键工具。然而，从样品制备到 数据呈现 的漫长征程中，无数科研人曾深陷条带消失、背景污浊、结果不可重复的泥潭。本文将基于核心实验流程，系统梳理WB实验中的高频“雷区”并提供切实可行的避坑方案