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DNase Test: Principle, Procedure, Results • Microbe Online Positive: When DNA is hydrolyzed, methyl green is released turning the medium colorless around the test organism Negative: If there is no degradation of DNA, the medium remains green It is used to differentiate S aureus (DNase +ve) from other Staphylococci that do not produce such enzyme
Deoxyribonuclease (DNase) Test - Procedure, Uses and Interpretation DNase agar is a differential medium that tests the ability of an organism to produce an exo-enzyme, called deoxyribonuclease DNase are extracellular endonucleases that cleave DNA and release free nucleotides and phosphate
DNase Test- Definition, Principle, Procedure, Result, Uses A positive test is demonstrated by the development of a pink or red halo around the colony or the well in the agar A negative test is demonstrated by no change in the royal blue color of the medium
DNASE TEST AGAR - Thermo Fisher Scientific Remel DNase Test Agar is a solid medium recommended for use in qualitative procedures to detect deoxyribonuclease (DNase) activity in microorganisms
33: Deoxyribonuclease (DNAse) Test - Biology LibreTexts Deoxyribonuclease (DNAse) agar actually has DNA in it Deoxyribonuclease is an exoenzyme excreted from the cell which will break the DNA down into smaller molecules There are 2 kinds of DNAse agar that can be used : The first medium has no indicator, but is does require HCl reagent to be added after growth has occurred on the plate
DNASE TEST AGAR WITH TOLUIDINE BLUE - cbsalife. com Enzymatic activity is detected by flooding the plate with 1 N hydrochloric acid (HCl) A clear zone surrounding growth indicates a positive reaction Soy Agar and subsequently reported a correlation between coagulase production and DNase activity
Microbiology - Merck For detecting microbial DNase (deoxyribonuclease) by the method of JEFFRIES et al (1957) and for identifying microorganisms, especially DNase-positive staphylococci This culture medium complies with the recommendations of the International Organization for Standardisation (ISO) (1977)
30787 Deoxyribonuclease Test Agar (DNase Test Agar) Deoxyribonucleic acid (DNA) can be hydrolysed by microorganisms producing DNase If the medium is then flooded with 1 N HCl not hydrolysed DNA precipitates (turbidity) and around DNase-positive colonies clear zones can be observed Sodium chloride maintains the osmotic balance of the medium and Agar is the solidifying agent