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11固氮酶基因nifH扩增引物评估 - 豆丁网 This problem occurs because a majority of nifH sequences have been generated using PCR primers that bind to conserved nucleotide positions found within the nifH gene A majority of the 393 full-length nifH sequences currently present in the nifH database are derived from sequenced genomes The two dips in nucleotide coverage (at
11固氮酶基因nifH扩增引物评估 - 道客巴巴 We performed an in silico analysis of the specificity and coverage of 51 universal and 35 group-specific nifHprimers by using an aligned database of 23,847 nifH sequences