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Picard Tools - By Broad Institute Use Picard Tools The tools, which are all listed further below, are invoked as follows: java jvm-args -jar picard jar PicardToolName OPTION1=value1 OPTION2=value2 See the Tool Documentation for details on the Picard command syntax and standard options as well as a complete list of tools with usage recommendations, options, and example commands
Tool documentation - Broad Institute Be sure to consult the Frequently Asked Questions (FAQ) page for useful information about typical problems you may encounter while using Picard tools
Picard Metrics Definitions - Broad Institute Picard A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM BAM CRAM and VCF View the Project on GitHub broadinstitute picard Latest Jar Release; Source Code ZIP File; Source Code TAR Ball; View On GitHub; Picard Metrics Definitions
Frequently Asked Questions - Broad Institute A: Most Picard tools can do this To read from stdin, specify dev stdin as the input file To write to stdout, specify dev stdout as the output file, and also add the option QUIET=true to suppress other messages that otherwise would be written to stdout
SAM Differences in Picard - Broad Institute A number of Picard tools can handle secondary alignments, but typically these tools either ignore these alignments or pass them from input to output unchanged MergeBamAlignment has the PRIMARY_ALIGNMENT_STRATEGY that can be used to determine how the program will select a primary alignment among multiple alignments for a segment in an aligner's
CollectInsertSizeMetrics (PICARD JDK API Documentation) - Broad Institute Field Detail Histogram_R_SCRIPT protected static final java lang String Histogram_R_SCRIPT See Also: Constant Field Values; Histogram_FILE @Option(shortName="H", doc="File to write insert size Histogram chart to ") public java io File Histogram_FILE; DEVIATIONS
DownsampleSam (picard 2. 10. 9-1-gaa8c979-SNAPSHOT API) - Broad Institute Class to randomly downsample a BAM file while respecting that we should either retain or discard all of the reads for a template - i e all reads with the same name, whether first or second of pair, secondary or supplementary, all travel together