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N-Ethylmaleimide (NEM) - Thermo Fisher Scientific Procedure or Protocol for Blocking Sulfhydryls on a Protein Note: Maleimides react with sulfhydryls at pH 6 5-7 5 to form stable thioether bonds At pH values > 7 5, reactivity toward primary amines and hydrolysis of the maleimide group can occur
Maleimide conjugation - protocols. gofflab. org Mix with 3 nmol of oligo for a conjugation ratio of 5:1 Incubate at room temperature O N Probably overkill but at least this opens more of our maleimide ring
Improving the Stability of Maleimide–Thiol Conjugation for Drug . . . Herein, we report on the detailed analysis of the postulated transcyclization reaction using a model compound system by HPLC‐MS measurements and investigated the stability in the presence of GSH Furthermore, the feasibility of this method was confirmed for a drug‐peptide conjugate
Conjugation Protocol for Thiol-Reactive (maleimide) dyes Since disulfides do not react with maleimides, it may be necessary to reduce these disulfide bonds prior to conjugation Conjugation is also reversible, so it is imperative that the buffer used is made and maintained between pH 7–7 5