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PCR controls - QIAGEN A no-template control (NTC) allows detection of contamination of the PCR reagents An NTC reaction contains all real-time PCR components except the template Detection of a positive signal in an NTC reaction indicates the presence of contaminating nucleic acids
Avoiding false positives with PCR | IDT In PCR experiments, amplification in the “no template control” (NTC) before the ~38th cycle with probe-based assays (or ~34th cycle when using intercalating dyes) is a sign of false positives and or contamination
Amplification of the No Template Control (NTC) If you get an amplification product in your no template control (NTC), review these possible causes and how they will appear in your plot
qPCR Terms Explained: Methods, Controls and Types | IDT The no template control (NTC) tracks contamination and primer-dimer formation that could produce false positive results (in the case of intercalating dye-based assays)
Controlling for contamination in qPCR testing - LGC The usual method to monitor for contamination in a qPCR assay is to use “no template controls” (NTCs) NTC wells contain all the qPCR reaction components, such as primers, master mix and water, but lack the sample nucleic acid template
Poxvirus PCR Diagnostic Protocol - Centers for Disease Control and . . . Negative PCR control (e g , no template control (NTC): control specimen containing all reagents except the target template; molecular grade water is an example of an appropriate NTC A NTC is a mechanism to detect cross-contamination of specimens
Controls in Real-Time Polymerase Chain Reaction Based Techniques Real-Time PCR enables the user to monitor the amplification of a deoxyribonucleic acid (DNA) or complementary DNA (cDNA) target during the PCR run, in real-time, and not at the end, as it is the case in conventional PCR
qPCR: Avoiding Signals in the No-template Control (NTC) I see amplification in the no-template control (NTC) reactions and unacceptable technical replicate differences in the Ct values I tried total RNA extraction and using Dynabeads from Invitrogen and prefer the latter because I get better-quality RNA as determined by Nanodrop and Bioanalyzer
PREVENT AND MANAGE AMPLICON CONTAMINATION IN YOUR LAB Nucleic acid amplicons are not infectious or hazardous to human health However, if not contained, amplicons can potentially contaminate surrounding surfaces and equipment, personnel and research experiments