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Probe-based bacterial single-cell RNA sequencing predicts . . . Here we developed probe-based bacterial sequencing (ProBac-seq), a method that uses libraries of DNA probes and an existing commercial microfluidic platform to conduct bacterial single-cell RNA
Microbial single-cell RNA sequencing by split-pool barcoding Single-cell RNA sequencing (scRNA-seq) has become an essential tool for characterizing gene expression in eukaryotes, but current methods are incompatible with bacteria Here, we introduce microSPLiT (microbial split-pool ligation transcriptomics), a high-throughput scRNA-seq method for Gram-negativ …
Identification of intracellular bacteria from multiple single . . . One increasingly popular approach for studying intracellular or host cell–associated microbes is the analysis of microbial reads from single-cell RNA sequencing (scRNA-seq) in the settings of viral infection or of cancer (17 – 21)
Ushering in a new era of single-cell transcriptomics in bacteria Abstract Transcriptome analysis of individual cells by single-cell RNA-seq (scRNA-seq) has become routine for eukaryotic tissues, even being applied to whole multicellular organisms In contrast, developing methods to read the transcriptome of single bacterial cells has proven more challenging, despite a general perception of bacteria as much simpler than eukaryotes Bacterial cells are harder
Bacterial droplet-based single-cell RNA-seq reveals . . . BacDrop is a droplet-based technology for single-cell RNA-seq in bacteria that can be scaled to millions of bacterial cells or hundreds of samples and was used to elucidate transcriptionally distinct bacterial subpopulations associated with varying phenotypic outcomes linked to antibiotic resistance and persistence
Bacterial droplet-based single-cell RNA-seq reveals . . . - PubMed We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome many challenges hindering the development of scRNA-seq in bacteria BacDrop can be applied to thousands to millions of cells from both gram-negative and gram-positive species It features un …
Bacterial single-cell RNA sequencing captures biofilm . . . Here, we present an optimized bacterial single-cell RNA sequencing method, BaSSSh-seq, to study Staphylococcus aureus diversity during biofilm growth and transcriptional adaptations following
Bacterial single cell RNA-seq reveals antibiotic resistance . . . While eukaryotic cells have been tested and validated extensively on single cell sequencing platforms, prokaryotic cells (in this case, bacteria) don’t follow the eukaryotic norms For example, one crucial difference between mammalian and bacterial cells is in the composition and accessibility of their messenger RNA (mRNA) molecules, or transcripts These molecules carry the coding sequences
Microbial single-cell RNA sequencing by split-pool barcoding Abstract Single-cell RNA-sequencing (scRNA-seq) has become an essential tool for characterizing gene expression in eukaryotes but current methods are incompatible with bacteria Here, we introduce microSPLiT, a high-throughput scRNA-seq method for gram-negative and gram-positive bacteria that can resolve heterogeneous transcriptional states We applied microSPLiT to >25,000 Bacillus subtilis
Single-cell RNA sequencing reveals plasmid constrains . . . Here, we use microbial split-pool ligation transcriptomics to study the relationship between bacterial subpopulation formation and plasmid-host interactions at the single-cell level