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The glycolytic enzyme phosphofructokinase-1 assembles into filaments The filament was formed from stacked tetramers that were related by a rotation of 221° and a translation of 83 Å between subunits (Fig 3, A and B) Although we did not directly determine the hand of the PFKL filament by EM, the PFK crystal structure only fit well in the right-handed helix
Phosphofructokinase - an overview | ScienceDirect Topics Phosphofructokinase (PFK) is defined as a key regulatory enzyme in glycolysis that catalyzes the irreversible transfer of a phosphate group from ATP to fructose-6-phosphate, with its activity being sensitive to pH and regulated by allosteric mechanisms including inhibition by ATP and citrate