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Is the full crossmatch valid after saline replacement Hi all, We have a patient with strong rouleaux and an alloantibody According to our policy, we need to perform a full crossmatch which means the crossmatch is carried from immediate spin, to 37C and to AHG phases Due to the strong rouleaux, a saline replacement technique has to be used during t
can you tell if Rouleaux is present just from looking at the gel card . . . I apologize, this may be a stupid question For example, if you are trying to do a gel cross match and the donor cells have Rouleaux, can you tell that from looking at the gel card? If so, can you tell me where I can find some pictures of this reaction Thank you for your time and help in advance
Rouleaux interference - Transfusion Services - PathLabTalk Expand It's not uncommon to see rouleaux interference in Gel testing but it's not something we see frequently The crossmatch question is interesting: Technically, the screen is negative for alloantibody reactivity so I think you'd be able to follow your procedure for that result However, with the immediate spin crossmatch, you may have to perform a saline replacement to obtain a "compatible
Rouleaux or cold auto - Transfusion Services - PathLabTalk We perform our antibody screens at 37C and IAT only (since AABB STD's says methods that detect SIGNIFICANT antibodies) The problem is, that crossmatches are performed at Immediate Spin Cold autos are missed at antibody screening, and the screen is reported as negative When techs crossmatch units,
saline replacement in serum discrepancy - PathLabTalk How do you perform saline replacement when there is serum discrepancy due to rouleaux formantion?From the last step,2 drops of saline is added and resuspend it with red cells does it need to read the agglutination macroscopically?(because iso-antibodies are IgM antibodies) AABB Technical manual d
anti-A1 or rouleaux? - Transfusion Services - PathLabTalk Almost certainly because it is anti-A1, rather than rouleaux, or any other "cold" antibody specificity, such as anti-ALeb, but, come what may, unless the reactions are positive at STRICTLY 37oC, it wi
Rouleaux in Ortho Gel Testing - PathLabTalk We are seeing rouleaux fairly frequently that appears in one screen cell and not the other, and in some of the gel panel cells the same way This is in a Ortho IgG gel card ran on a Vision analyzer The Rouleaux is verified in tube under the scope We normally run a PEG tube screen and panel on t
Rouleaux - Transfusion Services - PathLabTalk Is this a good enough, to prove that Rouleaux is causing the reactions in Gel? What would you recommend to indicate reactions are Rouleaux and not something else?
Rouleaux detected in IS crossmatch - PathLabTalk Our current crossmatch procedure requires that if we have reactivity at IS that is resolved with saline replacement (rouleaux) that we need to set up the tubes again and take testing through AHG phase If the patient has no history of clinically significant antibody and current antibody screen is
Do you wash your screening cells? For rouleaux? Why? We also would not consider discarding these products We transfuse about 25,000 rbc's a years, with a guess of 9% pos, we'd need to discard 3,600 units of blood, and eliminate them as donors I can't imagine that We do have certain groups of patients we give K neg to even if they don't have the