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Is the full crossmatch valid after saline replacement We have a patient with strong rouleaux and an alloantibody According to our policy, we need to perform a full crossmatch which means the crossmatch is carried from immediate spin, to 37C and to AHG phases Due to the strong rouleaux, a saline replacement technique has to be used during the immediate spin crossmatch phase
Do you wash your screening cells? For rouleaux? Why? 1) We did the gel card Ab screen and it showed rouleaux in the gel 2) we read manufacturer's procedure of ridding rouleaux by washing screening cells 6 times 3) after washing screening cells, we pipet them into the gel cards and finally add the patients plasma 4) incubate for 15 mins 5) centrifuge gel card 6) Rouleaux all gone
Dealing With Cold Agglutinins - Transfusion Services - PathLabTalk As far as the rouleaux not being an issue with the reverse typing, the Echo testing platform cannot reliably detect hemagglutination reactions that are graded as 1+ or less in test tube methodology The IS XM reactions with the example above were graded as W+ by the tech
can you tell if Rouleaux is present just from looking at the gel card . . . Rouleaux shows up in gel as either haze or mixed-cell (see the pictures) Haze = rouleaux (there's nothing else significant that causes this picture) Period Yes, in tube testing it's very hard to tell rouleaux from weak agglutination unless you use the microscope and or saline replacement This is not the case in gel Leave it, you are done
Rouleaux or cold auto - Transfusion Services - PathLabTalk EDTA tubes will have more rouleaux because of the higher protein level We also get surprizes, because we use gel, so never see tube reactions until we do IS XMs For patients with negative antibody screens who show 'stickiness' at IS (we also have a cold BB room), we examine for rouleaux, if not, we do a quick (5 minutes) 37C inubation
Rouleaux interference - Transfusion Services - PathLabTalk We recently had a positive antibody screen in gel, we ran a panel ( also in gel) and got some reactions but no pattern and everything was ruled out We then did an antibody screen in tube and saw that there was rouleaux at immediate spin but the screen was negative at AHG Is it common to have ro
anti-A1 or rouleaux? - Transfusion Services - PathLabTalk However, that 4+ reaction on the B cells cannot be due to the anti-A1 and I highly doubt that it is due to rouleaux; At least from my experience I don't typically see rouleaux this strong and we should have seen it with similar strength against the A1 cells I agree that you probably have a cold reactive allo antibody going on with the B cells
Microscopes in the Blood Bank - PathLabTalk If you use plasma instead of serum the increased protein can enhance rouleaux Sometimes it is difficult to differentiate rouleaux from true agglutination under the scope The novice general Med Tech (or the OCD Med Tech) tend to see two or three red cells "holding hands" under the scope and call this a W+micro reaction
How often do you see rouleaux when you are doing IS crossmatches? When I did my clinicals, the blood bank there would use the long pink tops with EDTA In my four weeks there, I saw rouleaux 10 times However this was a city of 200,000 people When I got my first job at a different hospital, the blood bank there was still using red tops Out of the whole year, I saw only two patients with rouleaux
Rouleaux - Reaction With Specific Cells on the Cell Panel I have never heard of rouleaux reacting with a specific antigen specificity (the two reactions, rouleaux and agglutination, are caused by different forces), but, of course, agglutination caused by certain antibody specificities (the Lutheran antibodies and anti-Sda, for example) appear very much like rouleaux :):)